Journal: Journal of Bacteriology
Article Title: Conjugative Transfer and cis -Mobilization of a Genomic Island by an Integrative and Conjugative Element of Streptococcus agalactiae
doi: 10.1128/JB.02199-12
Figure Lengend Snippet: Analysis of eight clones of transconjugants obtained after filter-mating experiments between donor strain 515 (carrying ICE_515_tRNALys) and recipient strain S. pyogenes ATCC 12202. (A) Amplification of the right junction of the ICEs in these clones as for donor cells, using primers specific of the integrase genes and of the tRNALys gene (lane D). The negative control is S. pyogenes ATCC 12202, used as recipient cells (lane R). Lane M shows DNA molecular weight marker. (B) Analysis of the transconjugants by pulsed-field gel electrophoresis in parallel to the donor strain (lane D) and the recipient strain (lane R).
Article Snippet: In addition, they have the same genetic background than the recipient S. pyogenes strain, as shown by pulsed-field gel electrophoresis ( ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig 3 caption a7 Analysis of eight clones of transconjugants obtained after filter-mating experiments between donor strain 515 (carrying ICE_ 515_tRNA Lys ) and recipient strain S. pyogenes ATCC 12202. (A) Amplification of the right junction of the ICEs in these clones as for donor cells, using primers specific of the integrase genes and of the tRNA Lys gene (lane D).
Techniques: Clone Assay, Amplification, Negative Control, Molecular Weight, Marker, Pulsed-Field Gel, Electrophoresis